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3 Summary of Genes and Proteins Important for NHEJ Mammalian Gene Name LIG4 XRCC4 XRCC5 XRCC6 PRKDC (formerly XRCC7) ARTEMIS Protein DNA ligase IV; cooperates with XRCC4 to ligate broken DNA molecules after their ends have been properly processed XRCC4; cooperates with DNA ligase IV to ligate broken DNA molecules after their ends have been properly processed Ku80; cooperates with Ku70 to bind DNA ends and recruit other proteins Ku70; cooperates with Ku80 to bind DNA ends and recruit other proteins DNA-PKcs; protein kinase activates Artemis Artemis; nuclease regulated by DNA-PKcs important for preparing DNA-PK ends to make them ligatable " 2007 by Taylor Francis Group, London.
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Ceramide -galactosidase hexosaminidase A ganglioside neuraminidase -galactosidase glucocerebrosidase GM1 Generalized gangliosidosis GM2 Tay-Sachs disease -galacto- sidase A Gauchers disease sphingo- myelinase hexosaminidase A and B Fabrys disease Sphingomyelin Niemann-Pick disease Globoside Sandhoffs disease Ceramide Ceramide Ceramide Ceramide GM3 Ceramide FIGURE 2 Electron micrograph of a portion of a brain cell from an infant with Tay-Sachs forex indicator predictor v2, showing predicto ganglioside deposits in the indlcator.
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Polypeptide HS react with FDNB; hydrolyze; separate amino acids reduce disulfide bonds (if present) SH cleave with trypsin; separate fragments; sequence by Edman degradation cleave with cyanogen bromide; separate fragments; sequence by Edman degradation 2,4-Dinitrophenylglutamate detected T-1 GASMALIK T-2 EGAAYHDFEPIDPR T-3 DCVHSD T-4 YLIACGPMTK C-1 EGAAYHDFEPIDPRGASM C-2 TKDCVHSD C-3 ALIKYLIACGPM establish sequence T-2 T-1 T-4 T-3 Amino terminus EGAA YHDFEPIDPRGASMALIKYLIACGPMTKDCVHSD C-1 C-3 C-2 Carboxyl terminus Amino acid sequence (protein) GlnTyrProThrIleTrp DNA sequence (gene) CAGTATCCTACGATTTGG 372 CHAPTER7.
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